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KMID : 0386819940040020001
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Journal of the Institute of Health Environmental Sciences
1994 Volume.4 No. 2 p.1 ~ p.9
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Amplification of a Variable Number of Tandem Repeats(VNTR) Locus(pYNZ22) by the PCR and Its Use For Evaluating Engraftment Following Bone Marrow Transplantation
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Abstract
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A genetic locus (Defined by DNA probe pYNZ22) that contains a VNTR has been amplified from a genomic DNA by the PCR. A normal unrelated persons and a series of bone marrow transplantation (BMT) patients with their HLA-identical siblings were
analyzed.
Amplification of the VNTR loci revealed the discrimination of 20 out of 26 unrelated persons and 5 out of 8 siblings. When the result of PCR was compared with RFLPs in the case of BMT candidates and their siblings, probe pYNH24 revealed the
discrimination of 4 out of 8 sibling pairs and p13-1-25 revealed the discrimination of 6 out of 8 sibling pairs. Three polymorphic genetic loci detected a donor and recipient DNA at least one or more loci among all 8 of sibling pairs. Above all,
the
studies of genetic locus that is apparent to individual identification are of practical value such as BMT which needs essential search. Both PCR and RFLP, molecularbiol work, are coming into wide use for polymorphism research. Thus the use of DNA
polymorphism between donor and recipient is very important and is easily applicable to clinical studies of BMT.
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KEYWORD
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